ABSTRACT
To establish the fingerprints of biles of pig, cattle and sheep, HPLC was used with Acclaim™ RSLC 120 C₁₈ column (3.0 mm×100 mm, 2.2 μm, 120 Å), the column temperature 35 °C, acetonitrile-1% perchloric acid as mobile phase, gradient elution, 0.5 mL·min⁻¹ flow rate, and detection wavelength at 200 nm. The fingerprint was generated by using Similarity Evaluation Software of Chromatographic Fingerprint of Chinese Medicine (2004A Edition). The fingerprint peaks were identified by reference substances and verified by ELSD and LC-MS/MS. Then, the biles of pig, cattle and sheep were detected to contain 14, 9 and 8 common fingerprint peaks respectively, and the similarity was greater than 0.92. To analyze each technical parameter, GHDCA in pig bile and TCA in cattle and sheep bile were selected as reference peak. The precision, repeatability and stability all meet the requirements of fingerprint establishment. The RSD of the relative retention time of the fingerprint peaks was less than 1.5%, and the RSD of the relative peak area was less than 5%. The fingerprint peaks in pig bile were THDCA, TCDCA, GHDCA and GCDCA, and TCA, TCDCA, GCA, GCDCA and GDCA in cattle and sheep bile. The main components of pig, cattle and sheep bile were conjugated bile acids, but there were significant differences in bile acids between pig bile and cattle, sheep biles. The HPLC method established in this paper is simple, rapid and reproducible, and could be applied to the identification and quality control of biles.
ABSTRACT
In this paper, herbal records and studies on pharmacological activity and chemical component of pig bile were reviewed for a systematic and comprehensive summary about this traditional Chinese medicinal material. Beyond that, not only national standards but also local standards for the quality control of pig bile were analyzed and summarized. It is indicated that this traditional Chinese medicinal material has a broad medicinal prospect, and the standards for quality control should be revised and improved.
Subject(s)
Animals , Bile , Chemistry , Medicine, Chinese Traditional , Quality Control , SwineABSTRACT
A LC-MS method was established for study of characteristic ions in frequently-used traditional Chinese medicinal materials derived from animal bile. UPLC-Q-TOF was used in the data acquisition work, then, the software of MarkerLynxTM v.4.1 was performed in the chemometric analysis of data. Besides, selected ion chromatograms of these bile acid ions were comparative studied. Better results were gained in the specificity identification of pig bile, bear bile, cultivated cow-bezoar , artificial cow-bezoar and some compound preparations(Hugan tablet and Rengongniuhuang Jiaxiaozuo capsule) by this method. The method is suitable for the specificity identification of pig bile, bear bile, cultivated cow-bezoar , artificial cow-bezoar and compound preparations containing these medicinal materials.
Subject(s)
Animals , Cattle , Bezoars , Bile , Chemistry , Chromatography, High Pressure Liquid , Ions , Chemistry , Mass Spectrometry , Medicine, Chinese Traditional , Species Specificity , Swine , UrsidaeABSTRACT
In ultrastructural level, this paper shows that the pig bile extracts and its effective constituent, the sodium deoxycholate, could fragment the Trichomonas vaginalis in vitro, The flagellum and caudal projection were splitted up; the plasma membrane, the intracellular membrane system, such as the limiting membranes of hydrogcnosome, phagosome and nuclear envelope were injured too; and cytoplasm was condensed, even the whole body was fragmented. The mechanism of the effeces is probably due to the fact that the surface activity of the drugs accelerated degradation of lipids which are made of the biomembrane system of human trichomonas vaginalis.